To identify the optimal condition for growth of Tetraspora in TAP medium, an initial OD was used to compare for S-curve of growth. The initial ODs were ranged from 0.005 to 0.2 and the culture density was monitored by measuring OD every 12 hours (Figure 3.5B). An initial OD of 0.01 showed the smooth pattern of S-curve. Then all further experiments used an initial OD730 of 0.01 when growing cells