The resulting ABTṠ+ radical solution was diluted properly with 0.2 mol/L phosphate buffer (pH 7.4) to
obtain absorbance at 734 nm of 0.70 ± 0.02. Next, 0.2 mL of mulberry leaf extract (with proper dilution) was added to 3.8 mL of ABTṠ+ solution. The mixture was mixed thoroughly and allowed to stand at room temperature in dark for 7 min.