Serial dilutions were made and 0.1 mL was spread over previously prepared plate count agar (PCA) or chloramphenicol glucose agar (CGA) and incubated at 5 1 C for 10 days or for at 25 1 C for 3 days for determination of psychrophilic bacteria or molds and yeasts, respectively. Plate counts were expressed as log10 CFU/mL.