the final quality ofpDNA preparations obtained with a process
that explores precipitation, hydrophobic interaction chro-matography and size exclusion was not significantly affected by strain genotype. Finally, high cell densityfed-batch cultures were performed with GALG20, this time using another ColE1-type plasmid, NTC7482-41H-HA, in pre-industrial facilities using glucose and glycerol. These experiments demonstrated theability of GALG20 to produce high pDNA yields of the order of 2100–2200 mg/L.