The obtained n-3 long chain (LC)-PUFA enrichment was not as efficient for all n-3 PUFA sources. The lowest enrichment efficiency (≈6%) was observed when flaxseed (α-linolenic acid source) was supplemented. Drastically higher n-3 LC-PUFA enrichment efficiencies were observed with supplementation of the n-3 LC-PUFA sources. However, for the n-3 LC-PUFA sources (fish oil, I. galbana and DHA Gold) differences in enrichment efficiencies were observed (≈55%, ≈30% and ≈45%, respectively), mainly because of different bio-accessibility of the n-3 PUFA and different n-3 PUFA profiles of the three sources.