his assay permits analysis of the motile cell behavior. (a) Schematics of the overall procedure. (b) Phase contrast photographs of the cultures taken at 0 h (immediately after scratching) and at the indicated time intervals show the wound closure by cells showing EMT (MDCK-Snai1) in a random migration manner (right panels) and as a cohesive sheet by epithelial cells (MDCK-CMV) (left panels). (c) Quantification of the wounded area invaded at different time points by MDCK-CMV (CMV; white) and MDCK-Snail1 cells (SNAIL1) represented as the percentage of wounded area at time 0. Scale bar = 20mum. Results represent the mean of four measures of each wounded area, obtained on duplicate biological samples (n = 8). Error bars indicate s.d.