The method for the determination of fatty acids profile of different
food samples has been optimized on two different cyanopropyl
columns. Results of the method validation showed that the method
has acceptable selectivity, linearity, precision, detection and quantitation
limits, robustness and stability. The obtained resolution of
FAMEs are better in comparison with data shown in references for
cis and trans isomers especially for elaidic and oleic acid. The method
is suitable for routine analysis of samples of vegetable and animal
origin with a very distinct fatty acid profile. Results may be
used for various purposes like nutritional labeling, quality control
of different food supplements containing specific fatty acid profiles
and confirmation of diet applied on animals. Better resolution of
trans/cis isomers was obtained with DB-23 column and it will be
used for the determination of trans fatty acid content in food.
DB-225ms column gave us confirmation of peak identity due to
different elution order of FAMEs and it is sufficient for the routine
analysis of vegetable oils.