For lipid production by C. curvatus, a 5-L fermentor (BiotronLiFlus GR) was utilized (Fig. 1). It was loaded with 2 L of mannitol supernatant and 1 L VFAs. The pH was controlled at 5.5 by an auto-matic pH controller (Model KB-250, K&B). Lipid production was aerobic, so air was supplied at 0.5 VVM using an aerator. Temper-ature was maintained at 30°C and the fermentor was stirred con-tinuously at 400 rpm.
2.4. Analytical method