Endophytic fungi in roots of para rubber were separated in total of 107 isolates. Among them, 22 isolates was found to have percentage of inhibition to P. botryosa more than 80%, and 4 most high efficient in suppression were S2P - 8 18, S2P - 8 82,S2P - 2 18, and S2P - 8 81. All of there four endophytic fungi isolates were tested for the efficiency in controlling P. botryosa fungi on para rubber. It was found that there was no significant difference in efficiency in inhibiting P. botryosa among there four endophytic fungi isolates. Their efficiencies were also comparable to that produced by 0.1% Metalaxyl, same concentration used by farmers. When the efficiency of the four endophytic fungi isolates in suppression of P. botryosa fungi on Para rubber tree was tested, it was shown that the isolates S2P13-1 and S2P31-2 had similar efficiency to 0.1% Metalaxyl. These results found in the present study suggest that these selected endophytic fungi isolates have high efficiency to suppress the growth of P. botryosa which caused severe disease in para rubber. This provides potential for development of bio-product from endophytic fungi to be an alternative non-chemical fungicides for farmers to use in Para rubber plantation.