2.4.2. Respiration and ethylene production
A slice from each treatment and grower was removed fromthe pouch
and carefully placed in a 69-mL container, flushed with ethylene- and
CO
-free air, sealed and left for 1 h at 20 °C. Two 1-mL samples were
removed and CO
2
and ethylene concentrations were measured using
gas chromatography as described elsewhere (Besada,Jackman,Olsson,
& Woolf, 2010). This measurement was repeated on the same samples
17 h after HPP treatment (holding tissue at 20 °C) to determine whether
a build-up of CO
2
2
or ethylene in the packaging before and after HPP treatment
might have given false measures of respiration rate. Concentrations
of CO
nmol kg
−1
2
s
and ethylene were expressed as μmol kg
−1
, respectively.
−1
s
−1
and
2.4.2. Respiration and ethylene productionA slice from each treatment and grower was removed fromthe pouchand carefully placed in a 69-mL container, flushed with ethylene- andCO-free air, sealed and left for 1 h at 20 °C. Two 1-mL samples wereremoved and CO2and ethylene concentrations were measured usinggas chromatography as described elsewhere (Besada,Jackman,Olsson,& Woolf, 2010). This measurement was repeated on the same samples17 h after HPP treatment (holding tissue at 20 °C) to determine whethera build-up of CO22or ethylene in the packaging before and after HPP treatmentmight have given false measures of respiration rate. Concentrationsof COnmol kg−12sand ethylene were expressed as μmol kg−1, respectively.−1s−1and
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