This is may be because a lower inoculums density level (1–3 mL) is not enough for the culture organism to promote the fungal growth resulting in longer lag phase. The reduction in the activity at higher inoculum density is because of the faster depletion of the available nutrients (Iqbal et al., 2011 and Patel et al., 2009). Optimum inoculum density is an important consideration for fermentation process since the accumulation of spores can inhibit growth and development of the culture organism (Nighojkar et al., 2006).