fluorescens: For production
of crude extracts with and without cells, two culture
media were compared. Both media contained a base
formulated (per liter) with glycerol (10 g), dibasic
potassium phosphate (1.5 g), magnesium sulfate (1.5 g),
but one of them enriched with 20 g of potato starch and
the other with 20 g of polypeptone. Cell concentration
was evaluated by counting in a Neubauer chamber after
24 h of culture time at 28°C. After culture medium was
selected, influence of pH (6.5, 7.0 and 7.5) and
temperature (25, 30 and 35°C) were evaluated;