10ml of 50% (NH4)2SO4 solution was added, vigorously shaken and allowed to settle. Then the upper layer was collected and the absorbance read in Pye Unicam double beam Spectrophotometer at 450nm against hexane as blank [24]
10ml of 50% (NH4)2SO4 solution was added,vigorously shaken and allowed to settle. Then the upperlayer was collected and the absorbance read in Pye Unicamdouble beam Spectrophotometer at 450nm against hexaneas blank [24]