To broaden our knowledge about the toxicity of metals in marine elasmobranchs, cannulated spiny dog-
fish (Squalus acanthias) were exposed to 20 M and 100 M lead (Pb). Since we wanted to focus on
sub lethal ion-osmoregulatory and respiratory disturbances, arterial blood samples were analysed for
pHa, PaO2, haematocrit and total CO2 values at several time points. Plasma was used to determine urea,
TMAO, lactate and ion concentrations. After 96 h, Pb concentrations were determined in a number of
tissues, such as gill, rectal gland, skin and liver. To further investigate ion and osmoregulation, Na+/K+-
ATPase activities in gill and rectal gland were analysed as well as rates of ammonia and urea excretion.
Additionally, we studied the energy reserves in muscle and liver. Pb strongly accumulated in gills and
especially in skin. Lower accumulation rates occurred in gut, kidney and rectal gland. A clear disturbance
in acid–base status was observed after one day of exposure indicating a transient period of hyperventilation.
The increase in pHa was temporary at 20 M, but persisted at 100 M. After 2 days, plasma Na and
Cl concentrations were reduced compared to controls at 100 M Pb and urea excretion rates were elevated.
Pb caused impaired Na+/K+-ATPase activity in gills, but not in rectal gland. We conclude that spiny
dogfish experienced relatively low ion-osmoregulatory and respiratory distress when exposed to lead,
particularly when compared to effects of other metals such as silver. These elasmobranchs appear to be
able to minimize the disturbance and maintain physiological homeostasis during an acute Pb exposure.
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