. (a) Schematic representation of the HRP activity test based on probing ABTS oxidation after magnetic separation [38,39], together with the expected results for (1) AbPCs
incubated with HRP (HRP/Ab-PCs) and (2) PCs modified with a control Ab as negative control (HRP/Ab Control-PCs). (b) Calibration curve of the HRP activity rate k as
determined with free HRP at different concentrations CHRP. The data were fitted by a lineal function: k [min1
] = 39.34 cHRP [lg/mL] 0.019; the regression coefficient R2
was equal to 0.999. (c) Results of the activity measurements of the purified samples (PC) and the supernatants of the last washes (w3) during PC purification. The activity
results from the first washes (w1) are shown in the Supporting Information. The resulting activity rates are enlisted in Table 1.