The concentrations of guanosine monophosphate (GMP), inosine
monophosphate (IMP) and glutamic acid (GA) were determined using
high performance liquid chromatography (HPLC) on an 1100 series capillary
LC system (Agilent Technologies, Englewood, CO; Steed, 2010).
Standard curves were generated in 1 M HCl at 0.25 mg/ml, 0.5 mg/ml