At first, the explants were kept in TDZ-free
maintenance medium (flowering non-inductive
medium) for 4 weeks and then transferred to VW
basal medium supplemented with 1.8 mM TDZ, 2%
sucrose, 0.4 mg/L thiamine, 100 mg/L myo-inositol
and 0.2% Phytagel (flowering inductive medium).