found no reduction in lesion growth when
T. viride and P. digitatum were inoculated simultaneously on
oranges, as the inoculation time of the pathogen increased, the
lesions were reduced (Díaz and Vila, 1990); whereas in the present
study there was no difference in lesion growth when the
antagonist was inoculated before, simultaneously or after the
pathogen in all treatments (Table 4). The highest reduction of
lesion growth occurred when T. viride was inoculated 24 h before
C. gloeosporioides (77.40%), while the lowest reduction occurred
when T. longibrachiatum was applied 24 h after the pathogen
(18.38%). This suggests that T. viride as bio-pesticide must be
applied pre-harvest to reduce loss during transportation and sale.
Piotrowska and Dorszewski (1996) report T. viride as one of the
most effective antagonists against Alternaria alternata on potato,
improved upon only by T. koningii. The effectiveness of T. viride
was confirmed by Nallathambi et al. (2009) who isolated the
superior strain T. viride CIAH240.