To further streamline the selection process,wedeveloped amultiplex
PCR-based method and agarose gel electrophoresis of the
resulting amplicons. Although our goal was to prove the presence
of the two different PM-resistant genes in the progeny, we used an
Rpv1-linked marker (VMC1g3.2) to follow the DM resistant gene.
Our results showed that the Rpv1- and Run1-linked markers frequently
cosegregate. The data illustrated that MAS offers a rapid
and accurate method to select hybrid genomes that combine multiple
loci in grape.