From the collection of documents, this research to study fungal contamination of aflatoxin in food and milk.The fungus aflatoxin to grow well at 25 to 38 C or high humidity.98 percent within 10 minutes from research studies found that how Competitive Eenzyme Linked Immunosorbant Assay (ELISA) and use. ANOVA statistical analysis yielded the most precision to 100 percent from all samples 92 sample mean 0.331 edge 0.264 ng / ml.