The aim of this work was the design and the structural and
functional characterization of different liposomal formulations
based on SPC with bioactive compounds (omega-3, omega-6, VE)
and VC for developing a functional food in pasteurized chocolate
milk. Essential fatty acids like linolenic acid (x-3) and linoleic acid
(x-6) are contained in SPC and in the formulations, the stearic acid
(SA) and the calcium stearate (CaS) were incorporated for stabilize
the lipid bilayer by increasing rigidity. Besides, addition of CaS
incorporated a mineral (calcium) that increases the nutritional
value. Oxidative stability was determined with the thiobarbituric
acid method. The size and the shape were analyzed by light scattering
and light microscopy, respectively. And the packing of membrane
was studied with two probes (merocyanine 540 and
Laurdan). All studies were performed with or without vitamins
and before and after pasteurization in order to have information
about the effect of the heat treatment. Pasteurization was applied
for preserve the liposomes. Also, encapsulation efficiency of FA was
determined after 72 h of dialysis in pasteurized formulations. The
experiments mentioned were made in a food model system in
order to avoid fluctuations in data due to other components ofthe food product. Finally, applicability in the product was studied
by the rheological behaviors of liposomes and by the sensorial
tests. The sensory evaluation in commercial chocolate milk, with
and without liposomes, was done by the overall acceptability with
hedonic scale and triangular tests with 40 and 78 potential
consumers, respectively.