A total of 29 bacterial isolates of Streptococcus pyogenes were isolated from infected tonsils after tonsillectomy for 19 patients with recurrent tonsillitis were employed for this study. The ranges of patients’ age group were from 4 to 14 years; 12 of them were female and 7 were male. Those were with a clinical history of at least five to six times of tonsillitis per year. Full informative history had been taken directly from patients and the information was arranged in detailed formula sheet with surgeon assistance. All patients subjected to tonsillectomy and the presented study extended from September, 2012 to January, 2013. Sixteen isolates of S. pyogenes were identified on the surface of tonsils, while 13 isolates were identified in the crypts of tonsils. Bacterial isolates of S. pyogenes were identified by standard methods. In brief, depending on the colonial morphology and β-hemolytic activity on 5% blood agar medium. In addition to some of biochemical tests specially bacitracin discs (0.04 units, Becton, Dickinson and company spark, MD (TAXO) BD, USA) were used for identification of S. pyogenes (Goldman and Green, 2009). Isolated bacteria were maintained for intermediate storage in brain heart infusion broth with 20% glycerol in -20°C