The MeOH-soluble epidermal powder
(2.0% yield, wlw, from the air-dried
leaves of Chr, sempewirens) was scraped off
from the leaf underside (Figure 1). The
extract was purified on an hplc with an
ods column to yield three major compounds:
1 (55.2% from the MeOH extract),
2 (18.0%), and 3 (6.8%). These
three compounds in the samples collected
in California and Oregon were
distributed in a similar ratio.