The rates of hatching of the embryos transferred the cultured PGC, frozen–thawed cultured PGC
and freshly collected PGC were 28.0% (121/432), 30.6% (11/36) and 27.8% (20/72), respectively, and 57, 5 and 8 putative chimaeric chickens matured sexually and test mating was performed (Table 1).