The PCRs were performed in a 50-mL volume containing 10 ng of
fungal DNA, 0.2mmol l1 each of dNTP, MgCl2 0.16mmol l1,1 PCR
buffer (Mg2þ free), 0.8 mmol l1 each primer (ITS1:50- TCC GTA GGT
GAA CCT GCG G-30 , ITS4: 50-TCC TCC GCT TAT TGA TAT GC-30) (White
and Bruns, 1990) and 1U Taq DNA polymerase [Reagents were from
Po Bio-engineering (Dalian) Co., Ltd., Dalian, Liaoning Province,
China].