The advantages of the method include that it is highly sensitive, is able to measure 1-20 µg of protein and is very fast. Only relatively few materials interfere with it (it works even in presence of urea or guanidine hydrochloride) but, importantly, detergents do. Even traces of detergent (e.g. cleaning products) can invalidate the results. Its disadvantages are that it depends strongly on amino acid composition and that it stains the cuvettes used.