before 50 μL sodium methoxide was added. The reaction mixture was placed in a Thermomixer R (Eppendorf AG, Hamburg, Germany) for 90 min at 21 °C and 1000 rpm. In order to neutralize the catalyst and stop the reaction, 50 μL of 25% HCl was added. Samples were centrifuged and the upper hexane layer containing the fatty acid methyl ester (FAME) extract was transferred to a gas chromatography vial for analysis (Velasquez-Orta et al., 2012)