Only oocytes with a diameter P 110 lm were selected for IVM.
After reaching maturation, all oocytes were incubated with calcein-AM and ethidium homodimer-1 for an assessment of oocyte viability and Hoescht 33342 for an evaluation of chromatin
configuration (Fig. 4).
Table 3 shows the changes in chromatin configuration of oocytes cultured in absence or presence of EGF 50 or 100. The recov ery rates of viable oocytes P 110 lm in diameter did not differ between the treatments. However, the percentage of oocytes that resumed meiosis was significantly higher for the EGF 50 in
comparison to the other treatments (P < 0.05).