Reproductive seasonality in buffalo (Bubalus bubalis) is characterized by behavioral,
endocrine, and reproductive changes that occur over distinct periods of the year. During
the nonbreeding season (spring and summer), the greater light-dark ratio (long days)
suppresses estrus behavior and the occurrence of ovulation. Anestrous buffaloes have
insufficient pulsatile of LH to support the final stages of follicular development, and subsequently,
estrus behavior and ovulation do not occur, limiting reproductive efficiency,
especially in artificial insemination (AI) programs. A number of therapeutic strategies
designed to synchronize follicular wave emergence and ovulation have allowed for the use
of AI throughout the year, overcoming seasonal anestrus in buffalo. These therapies also
improve reproductive performance by increasing the service rate and pregnancy per AI in
buffalo herds, regardless of reproductive seasonality