Silica precipitation induced by silafÞns
(14). (A) Low molecular mass fraction of HF extract
from C. fusiformis cell walls. The extract was
subjected to TricineÐSDS-PAGE (13) and stained
with Coomassie blue. (B) Correlation between
silafÞn concentration (27) and the amount of
silica precipitated from a silicic acid solution. The
dotted line represents the result obtained for the
silafÞn mixture; the solid line shows the result for
pure silafÞn-1A (15). (C and D) SEM micrographs
of silica precipitated by silafÞn-1A (C) and the
mixture of silafÞns (D). The diameter of silica
particles is 500 to 700 nm (C) and ,50 nm (D).
The protein concentration was 5 mg/ml. Bar: 1
mm.