Granulosa cells from small (< 2mm) antral porcine follicles were grown in culture to study the effects of various hormones on growth, morphology and progesterone secretion. Culture medium 199D + 4% serum was found to be most suitable since it maintained a fairly constant cell population. Estradiol (lug/ml) and human FSH stimulated cell growth. LH and FSH stimulated progesterone secretion and induced morphological changes associated with luteinization. Estradiol (0.1ng/ml) inhibited progesterone secretion by granulosa cells.
PDF
Influence of Serum, Estrogen, and Gonadotropins Upon Growth and Progesterone Secretion by Cultures of Granulosa Cells from Small Porcine Follicles