The quality of cryopreserved rainbow trout sperm is low, so the number of cryopreserved spermatozoa required for successful fertilization is about 10 times higher than that for fresh semen . Low fertilization ability of cryopreserved semen is accompanied by low post-thaw motility . The low quality of cryopreserved semen negatively impacts rainbow trout breeding efforts. Therefore, improved techniques for rainbow trout sperm cryopreservation are needed.