Tobacco homogenates, 10 g (1 g tobacco + 9 mL of water) and 20 g (2 g tobacco + 18 mL of water), fortified with the pesticide mixture (100 ng/g), were extracted in separate batches (n = 6) with 10 mL EtOAc, followed by d-SPE cleanup using 150 mg PSA + 150 mg C18 + 75 mg GCB + 300 mg MgSO4 for 10 g and proportionately double amounts for 20 g. The recoveries were statistically compared.