Preparation of a sterile transfer chamber and equipment
A classroom transfer chamber can be made from a clean glass aquarium turned
on its side. Scrub the aquarium thoroughly with a 30% bleach solution, making
sure that you wear gloves and do not inhale the fumes. Rinse with sterile
distilled water, turn upside down on a clean counter or paper towels and allow
to dry. Cut holes in a clean plastic sheet to allow arms to reach into the
chamber and reinforce the cut edges with tape if necessary. Tape the clean
plastic sheet over the open side of the aquarium making sure that the arm
holes are located at a convenient height. Plastic sleeves could also be fitted to
these holes if you wish to make it easier to prevent the entry of airborne spores
into the chamber. The finished aquarium chamber can be sterilized by
spraying with 10% chlorox bleach just prior to each use and drying with sterile
paper towel.