initial denaturation step for 4 min at 94 C, followed by 36 cycles at 94 C for 30 s (denaturation), 46–56 C for 45 s (annealing) and 72 C for 120 s (extension), followed by a final extension step at 72 C for 7 min. DNA amplification reactions with SRAP primers were performed under the following cycle program