for a period of 16h, 20h,
24h, and 28h respectively. Then the curd was mixed
properly to break large curd particles and centrifuge at
10,000 rpm for 10min at 4°C using Kubota high speed
centrifuge (Japan). The supernatant was carefully decanted
and used for further analysis. The supernatant obtained
after centrifugation and filtration was lyophilized and used
when required