The P-2 fraction obtained in the TOYOPEARL CM-650M column chromatography was subjected to gel filtration through Superdex 200 10/300 GL. Fractions 14–16 were pooled as the purified AkEG21.
SDS-PAGE for cellulase preparations from A. kurodai is shown in the inset. Mk, marker protein; P-2, the sample after TOYOPEARL CM-650M chromatography; Fr. 15, purified AkEG21.