To determine the persistence of the treatment effect over time,
after the first set of inoculations, all the leaves were removed to
force a new flush. Shoots were allowed to grow another 2 weeks
until 4e6 new leaves developed. When the leaves achieved the
requisite size, a second set of inoculations was performed (5 weeks
after initial treatment) without an additional chemical application.
The experiment was conducted three times with six replications
per treatment.