Gamma irradiation (50-150 Gy) was most efficient followed by 0.05-0.10 % EMS and their combination treatment in inducing wide array of macromutation in tomato. Five putative mutants with exserted stigma flower, dark green fruit, dwarf plant having pyriform fruit from Patharkutchi, multiparous cyme from EC620177 and chlorophyll deficient mutant from EC620176 that could be isolated in M2 generation hold promise for their utilization in tomato breeding programme.