The extracts were then
combined and volume was concentrated in a rotating evaporator for removal of alcohol. MDW
in the ratio (0.3:4:1 v/v/v), Sample tubes fed with the mixture were loosely capped, placed in a
water bath for 5s, and left at room temperature for 10’ and taken in separating funnel, agitated
vigorously by occasional release of pressure, two phases separated. The organic phase was
discarded which removes the organic impurities and the methanol: water phase was assayed
for sugar. The residues were oven-dried at 50°C (overnight) to remove the residual solvent, and
stored at –2°C for analysis.