In all the 24 different combinations of growth regulators tested, the explants invariably showed emergence of a bud at the nodal regions of the multi nodal explants in 20 days. The concentration of BAP could be varied from 0.5 to 3.0 mg.L-1 without significant positive effects, showing the lower influence of PGR on nodal explants of N. khasiana for multiple shoot induction. The results obtained in the present study were in agreement with that of Latha and Seeni (1994), although the treatments were different. They reported that seedling derived nodal explants of N. khasiana could be induced to undergo bud proliferation from the node. The results of the present study further revealed that the response of the in vitro shoot culture was not particularly different from the seedlings. Generally, the blackening of the basal portion of the nodal explants observed in different nodal explants. Cultures did not affect the in vitro auxiliary branching of N. khasiana. However, exudation resulting in browning/blackening of the medium did not occur in most cases. Redwood and Bowling (1990) and Rathore et al. (1991) reported the synergistic effect of BAP and auxins on maximum shoot multiplication in Nepenthes