DPPH (2, 2-diphenyl picryl hydrazyl) is a commercially available, commonly used, stable free radical, which is purple in colour. Antioxidant molecules when incubated, reacts with DPPH and converts it into di-phenyl hydrazine, which is yellow in colour. The degree of discoloration of purple to yellow was measured at 520 nm, which is a measure of scavenging potential of plant extracts. 5 μl of plant extract was added to 195 μl of DPPH solution (0.1mM DPPH in methanol) in a microtitre plate. The reaction mixture was incubated at 25 0 C for 10 minutes, after that the absorbance was measured at 520 nm. The DPPH with corresponding solvents (without plant material) serves as control. The methanol with respective plant extracts serves as blank. The DPPH radical scavenging activity of the plant extract was calculated as the percentage inhibition.