MATERIALS AND METHODS
Plant Material
The experimental material consisted of two sets of germplasm. The first group comprised of the 40 Pakistani elite wheat cultivars (Table 1), whereas the second group comprised of 62 entries of National Uniform Wheat Yield Trial (NUWYT)nursery of 2 crop seasons 2008-2009 and 2009-2010 for both rainfed and irrigated categories. These categories of rainfed and irrigated denote the two major areas of wheat production based on the available source of water. Among the 40 elite cultivars germplasm, wheat genotypes were randomly selected for the determination of the 1BL.1RS translocation.