The AmB layer in contact with the d-lipids – sterol bilayer is thicker (47 ± 2) Å than on the h-lipid-sterols bilayer, but contrary to the hydrogenous membrane, the mainly less polyunsaturated
deuterated membrane becomes (3 ± 1) Å thinner in the chain region. Less AmB (1.6 ± 0.2) % v/v) also inserts in the deuterated membrane, leading to only very modest changes in the scattering length density of the chain region – from (6.61 ± 0.02) × 10−6 Å−2 to (6.55 ± 0.02) × 10−6 Å−2 in H2O, (6.54 ± 0.03) × 10−6 Å−2 in 66% D2O and (6.55±0.05) × 10−6 Å−2 in 100% D2O. The membrane thinning indicates that AmB prefers a smaller hydrophobic thickness than the original dlipid
– ergosterol bilayer (32 ± 1) Å, and causes the lipids to adopt a more disordered conformation, whereas in the more polyunsaturated hlipid- ergosterol membrane is able to accommodate AmB without a significant structural rearrangement.