. After the completion of sedimentation, indicated by the clustering of opaque nanoparticles on tube bottoms, viable spermatozoa were carefully recovered from supernatant by pipetting into appropriately labeled 50 mL tubes.
. After the completion of sedimentation, indicated by the clustering of opaque nanoparticles on tube bottoms, viable spermatozoa were carefully recovered from supernatant by pipetting into appropriately labeled 50 mL tubes.