However, these studies investigated only crush site, but do not study in DRG, proximal stumps, and distal stump. Moreover, they were not inquired autophagy was increasing at different time intervals and then defective to evaluate morphology of nerve regeneration because the size and number of axon not evaluated by a good method analysis. Thus, it is necessary to continue research into this topic. The present study aims to examine the alterations are apparent for autophagy in the dorsal root ganglia and peripheral nervous system after nerve injury.