The PCR products of the first juvenile group
showed electrophoretic patterns with both two and three bands
(Fig. 5(a)). Therefore, we inferred that the first group contained a
mixture of E. sinensis and E. japonica. Then, we conducted further
sequencing analysis of the first seed group to identify them to
species. Samples giving two and three bands were E. sinensis and
E. japonica, respectively (data not shown).