2.2. pH Determination
For the pH determination, 50 grams of sample were used and 20 ml of distilled water were added as described by BRASIL [9], and the reading was performed using a po- tentiometer (Mettler Toledo, mod DL 25).
2.3. Determination of Acidity
The method used was based on the extraction of fat using a mixture of ethyl ether: ethyl alcohol 2:1, with continu- ous stirring for 2 hours or 8 hours of rest and neutraliza- tion titration with NaOH 0.1 mol/L and phenolphthalein as an indicator, and the values were expressed as mg of NaOH/g of fat [10].