The 0.2 lm membrane gave the highest permeate flux, total vitamin C content, total phenolic content and antioxidant capacity as well as the highest value of irreversible fouling. Based on these results, the membrane with pore size of 0.2 lm was considered to be the most suitable membrane for the clarification of pineapple juice. The optimum operating conditions for the clarification pineapple juice by membrane filtration was a cross-flow velocity of 3.4 ms1 and transmembrane pressure (TMP) of 0.7 bar. An average flux of about 37 lm2 h1 was obtained during the microfiltration of pineapple juice under the optimum conditions using batch concentration mode.