In this study the screening process involved collecting algae samples from Dairy farm wastewater plant, and screening on the basis of NR fluorescence analysis provided successful way to select strains for determining microalgae cellular lipid content to form consortium. The dairy farm wastewaters could support the growth of consortium of native algae strains isolated from dairy farm wastewaters good for the production of biodiesel as well as possessed high wastewater nutrient removal efficiency. A consortium of 10 native strains produced maximum biomass and algal oil in treated wastewater at 10% CO2 supplement. The bio-volume ratio of microalgae was regulated at high CO2 concentration and breakthrough a new research for Ankistrodesmus sp., Chlorella strains, and Scenedesmus strains to culture for CO2 sequestration. Biomass and algal oil produced by consortium in HRAP was 153.54 t ha−1 year−1 and 29.47 thousand L ha−1 year−1, respectively.
This study revealed the capability of consortium of native strains supported by nutrients present in treated dairy farm wastewater to produce biodiesel and can remove >98% COD as well as nutrient very efficiently within 4–5 days of culture.